Research Associate /Sr Research Associate – Cloning /Molecular Biology
Job Code : CMBRA-0027-18
Location: Bedford, MA
We are seeking a Research Associate/Sr Research Associate for the Drug Discovery Biology Group at **MEMBERS ONLY**SIGN UP NOW***.. The individual will be energetic, self-motivated with a desire to succeed at the highest level, joining a team of scientists aiming to develop unique AAV/Gene Editing technology for targeted gene therapy indications. The individual will interact with other team members to design and execute advanced cloning technology to support on going molecular and cell biology experiments aimed at developing effective vector designs for appropriate biological targets. Significant cross-functional collaboration with a talented and dedicated group of scientists including molecular biologists, cell biologists, bioinformaticians and virologists will ensure a diverse exposure to the drug discovery process. As part of the team the candidate will have significant responsibility to drive success of their own project with opportunities to acquire new skills in an innovative and fast-paced environment. The position offers the opportunity to leverage our unique technology in early discovery projects and play a crucial role in the rapid translation to both pre-clinical and clinical settings.
Principal Duties and Responsibilities
Developing, optimizing and performing advanced cloning and genomic construction techniques to enable the characterization of the mechanism of action of HMI’s proprietary AAV gene editing technology.
Use advance cloning techniques such as Gateway, Golden Gate and Gibson Assembly in a medium and high throughput applications.
Work with the latest gene editing software packages (SnapGene / Vector NTI ) to assist in sequence analysis, and genomic predictions
Perform laboratory experiments and report data and suggest future work to management and project teams
Design and excecute a variety of mammalian cell based assays to confirm and evaluate both gene expression and gene editing vector designs.
Maintenance of electronic laboratory notebook including accurate and detailed recording of methods, resuls, data analysis, and conclusions
BS/MS or equivalent;
2-4+ years of industrial/academic laboratory experience
Proven ability to work independently and design experiments, trouble shoot, interpret and report data back to teams
Proficiency in cutting edge molecular techniques including Gibson assembly, large and small scale plasmid purification, DNA sequencing/analysis, and working knowledge of advanced molecular techniques and concepts
Experience with cell biology including maintenance of mammalian cell cultures, transient plasmid transfection, and Western blotting.
Familiarity with medium and high-throughput cell & molecular based assays and knowledge of AAV gene therapy a plus
Knowledge of DNA analysis software (SnapGene, Vector NTI, Geneious, Sequencher or similar)
Highly analytical mind, attention to detail, and organization skills are critical
Collaborative and proactive attitude
Excellent written and oral communication skills
Interested candidates should forward a cover letter and Resume / CV to careers-at-homologymedicines.com
******MEMBERS ONLY**SIGN UP NOW***., Inc. (HMI) has built foundational intellectual property on gene editing and gene therapy vectors derived from naturally occurring human adeno-associated viruses (AAVs). The Company's technology is precise, on-target and highly efficient for in vivo editing of genetic mutations. This transformative platform technology, combined with an elite leadership team of biopharmaceutical industry veterans, create a significant opportunity for HMI to rapidly advance a diverse pipeline of new medicines that address and potentially cure the underlying cause of genetic diseases
Job Type: Full-time