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Job Details

Senior Research Associate

Company name
Bristol-Myers Squibb Company

Location
Redwood City, CA

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Profile

Senior Research Associate Bristol-Myers Squibb is a global biopharmaceutical company whose mission is to discover, develop and deliver innovative medicines that help patients prevail over serious diseases. One shared journey is moving us forward at Bristol-Myers Squibb. Around the world, we are passionate about making an impact on the lives of patients with serious disease. Empowered to apply our individual talents and ideas so that we can learn and grow together. And driven to make a difference, from innovative research to hands-on community support. Bristol-Myers Squibb recognizes the importance of balance and flexibility in our work environment. We offer a wide variety of competitive benefits, services and programs that provide our employees the resources to pursue their goals, both at work and in their personal lives. The Senior Research Associate will join the Immuno-Oncology Resistance Biology group at Bristol-Myers Squibb in Redwood City, CA and aide in the advancement of early stage therapeutic targets at the intersection of tumor genetics and tumor immunobiology. The position will utilize cellular and molecular biology to engineer and characterize cells expressing or suppressing gene expression through recombinant methods. Engineered cells and cell lines will be profiled for validated expression as well as in vitro functionality of the target(s) through existing or newly-developed assays. The position will also entail operating a next generation sequencing lab. The position will require the individual to be highly collaborative and work closely with and support an array of project teams and researchers within the Discovery Research organization. Responsibilities General molecular biology involving isolation, manipulation, and characterization of DNA, RNA and proteins Next generation sequencing library preparation and sequencing Design of mammalian cell expression vectors for recombinant proteins, inhibitory RNAs, and CRISPRs Mammalian cell culture including hematopoietic cells and cell lines, including those of rodent or human origin Mammalian cell manipulation including transfection or lentivirus transduction of expression, interfering RNA, or CRISPR vectors Assessment of RNA and protein expression using qRT-PCR, flow cytometry, ELISA, HTRF or western blotting as appropriate Cell cloning and freezing Initial cell-based functional assay development and validation of newly engineered cells Detailed, formal record keeping through electronic notebook Communication of work, plans and progress using written and verbal communication in small and group settings Qualifications A B.S. with a minimum of 5 years of experience, or an M.S. with a minimum of 3 years of academic/industrial experience in molecular biology is required. Experience with a wide range of in-vitro cellular and biochemical assays, including cell culture, western blotting, immunoprecipitation, ELISAs (MSD), reporter assays, siRNA/shRNA knockdown, CRISPR-mediated genome editing, qPCR, cloning, lentivirus generation and transduction and the ability to incorporate state-of-the-art techniques to enhance productivity would be highly desired. Flow cytometry experience is a plus. Demonstrated experience in design and construction of cell expression plasmid constructs, site-directed mutagenesis, and recombinant protein vector design Extensive cell culture techniques including demonstrated ability to establish transient/stable cells and cell lines through transfections and/or virus transduction. Experience in work with primary human or rodent cells is desired. Proficiency in utilizing scientific software such as GraphPad, FlowJo, Cytobank , Spotfire, etc. for data analysis and figure generation is highly desired. Ability to multitask among multiple projects and teams and work independently in a fast-paced, highly interactive environment Demonstrated independence in experimental design, execution, data analysis and troubleshooting is required. Attention to detail with excellent organizational and record keeping skills is a requirement. Excellent interpersonal skills with the ability to interact effectively with people, internally and externally is required.

Job Type

Permanent

Job Reference

R1503886

Job ID

19434563

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Company info

Bristol-Myers Squibb Company
Website : http://www.bms.com

Company Profile
Bristol-Myers Squibb is a global BioPharma company firmly focused on its Mission to discover, develop and deliver innovative medicines that help patients prevail over serious diseases.

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